Bodipy cholesterol thermo
WebTopFluor® Cholesterol or Bodipy Cholesterol (878557-19-8) is a fluorescent sterol, used as a cholesterol marker and aids cholesterol trafficking. Buy now. US EN. Applications …
Bodipy cholesterol thermo
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WebCholesteryl Bodipy Fl C12, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results … WebThe green-fluorescent BODIPY FL C 5 -ceramide ( D3521) is more fade-resistant and brighter than the NBD derivative and can likely be substituted for the NBD C 6 -ceramide in many of its applications. The red-fluorescent BODIPY TR ceramide ( D7540) has proven useful for two-color immunofluorescence using a fluorescein-labeled antibody.
WebBODIPY™ 493/503 staining Thermo Scientific CAT: D3922 Lipofectamine 2000 Thermo Scientific CAT: 11668019 Pierce™ BCA Protein Assay Kit Thermo Scientific CAT: 23227 ... Bioengineering Institute CAT: A110-1 Total cholesterol (TC) Nanjing Jiancheng Bioengineering Institute CAT: A111-1 D-Glucose Sigma-Aldrich CAS: 50-99-7 Hoechst … WebApr 12, 2024 · Cellular lipid droplets were stained with 0.38 µM BODIPY 493/503 (ThermoFisher Scientific) for 25 min. Nuclei of the cells were stained with 0.38 µM DAPI (Roche) for 20 min. Microscopy was performed using a Leica SP8 confocal microscope (Leica Microsystems Inc.) with spectral detection and a Leica HC FLUOTAR 25x/NA 0.95 …
WebProduct Description. BODIPY 480/508-cholesterol is a biologically active and cell-permeable analog of cholesterol that is tagged with a fluorescent BODIPY group at … WebBODIPY Dyes Product Line BODIPY™ Shipping Condition Room Temperature Molecular Formula C14H17BF2N2 Physical Form Solid Product Type Fatty Acid Recommended …
WebThe orange-red fluorescent fatty acid, BODIPY™ 558/568 C 12 can be used as a synthetic precursor to a wide variety of fluorescent phospholipids. Unlike fluorophores like pyrene, DPH and NBD, BODIPY™ dyes are relatively environment insensitive and is fluorescent in both aqueous and lipid environments.
WebCholesterol is an abundant and important lipid component of cellular membranes. Analysis of cholesterol transport and diffusion in living cells is hampered by the technical … sow the wind and reap the whirlwind什么意思WebNov 28, 2016 · For BODIPY staining, after 24 h of seeding, the BODIPY reagent was added and incubated in the incubator for 30 min. After incubation, cells were washed with 1X PBS and observed under the confocal microscope (497 nm excitation and 503 nm emission). Image-Pro and GraphPad prism software were used to quantify the images and analyze … team nb gnbWebTo stain neutral lipids, cells were incubated with BODIPY (1:1,000; Thermo Fisher Scientific) for 1 h at 37 °C. Actin was stained with phalloidin (1:50; Invitrogen) for 30 min at RT and nuclei with DAPI (1:5,000; Thermo Fisher Scientific) for 15 min at RT. Samples were washed with PBS between steps. sow the wind 2020WebMar 1, 2024 · Cholesterol is a structural component of cell membranes. Most cells are incapable of its catabolism, and intracellular cholesterol accumulation is linked to several disorders including... team nature switzerlandWebUpon injection into the yolk sac, BODIPY-cholesterol did not disturb zebrafish development and was targeted to sterol-enriched brain regions in live fish. We conclude that this new probe closely mimics the membrane partitioning and trafficking of cholesterol and, because of its excellent fluorescent properties, enables the direct monitoring of ... team navasota used carsWebThe bis-BODIPY phospholipase A substrate—bis-BODIPY FL glycerophosphocholine (bis-BODIPY FL C11-PC, B7701 )—has been specifically designed to allow continuous monitoring of phospholipase A action and to be spectrally compatible with argon-ion laser excitation sources. sow the windWebThermo Fisherce bodipy cholesteryl 4 4 difluoro 5 4 methoxyphenyl 4 bora 3a Ce Bodipy Cholesteryl 4 4 Difluoro 5 4 Methoxyphenyl 4 Bora 3a, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more sow the wind and reap the whirlwind meaning