H2ax primer
WebJan 7, 2024 · Western blot analysis and γ-H2AX foci assay also revealed that targeting CXCR4 enhanced the expression of γ-H2AX (Fig. 2C), and caused the persistence of γ-H2AX foci formation upto 2 h ... WebMar 21, 2024 · H2AX (H2A.X Variant Histone) is a Protein Coding gene. Diseases associated with H2AX include Nijmegen Breakage Syndrome and Ataxia-Telangiectasia. …
H2ax primer
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WebApr 21, 2010 · We have developed a human cell line that permits induction of multiple DSBs randomly distributed and unambiguously positioned within the genome. Using this system, we have generated the first genome-wide mapping of gammaH2AX around DSBs. WebThe primer sequences were as follows: H2AX, F-AGCTGCTGGGCGGCGTGACG and R-CTCCTGGGAGGCCTGGGTGG; VP1, F-AGCACCCACAGGCCAGAACACAC, and R-ATCCCGCCCTACTGAAGAAACTA; GAPDH, F-GCAAATTCCATGGCACCGT and R-TCGCCCCACTTGATTTTGG. The changes relative to the levels of GAPDH were …
WebMA1-2024 was used in IHC to successfully detect H2A.X pSer140 in postnatal mouse lung section. MA1-2024 detects human and mouse phosphorylated H2AX. MA1-2024 has been used successfully in Western blot, immunofluorescence, and ELISA procedures. By Western blot this antibody detects ~17 kDa protein representing phosphorylated H2AX in gamma ... WebHistone H2A.X Antibodies Antibodies that detect Histone H2A.X can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry and ELISA. These antibodies target Histone H2A.X in Human, Mouse, Rat, Zebrafish and Ovine samples.
WebJan 1, 2015 · Phosphorylation on serine 139 of histone H2AX (γ-H2AX) is associated with DNA double strand breaks (DSBs) [1]. Its amplification and signalling function in recruiting other repair factors to the site of damage and its removal after damage repair is completed, makes γ-H2AX the most sensitive biomarker used to study DNA DSBs. WebSep 25, 2008 · Histone H2A variant H2AX is a dose-dependent suppressor of oncogenic chromosome translocations. H2AX participates in DNA double-strand break repair, but …
WebAug 12, 2011 · Normal cells, both in vivo and in vitro, become quiescent after serial cell proliferation. During this process, cells can develop immortality with genomic instability, although the mechanisms by which this is regulated are unclear. Here, we show that a growth-arrested cellular status is produced by the down-regulation of histone H2AX in …
WebOct 24, 2024 · Taken together, these data indicate that the phosphorylation of H2AX S139 is required for the direct interaction of H2AX with cGAS and facilitates the recruitment of cGAS to DNA-damage sites.... homemade stove top cleaner for gas stovesWebPrimer Pairs H2ax Mouse qPCR Primer Pair (NM_010436) H2ax Mouse qPCR Primer Pair (NM_010436) Product Code MP205900 Skip to the end of the images gallery Skip to the beginning of the images gallery $170.00 Qty Add to Cart Request Quote for Bulk Pricing Shipping Information Delivery charges will be calculated during checkout View Ordering … homemade storm shelter ideasWebFeb 16, 2024 · Here we focus on the histone variant H2AFX (commonly known as H2AX, further referred to as H2A.X), which represents about 2–25% of the cellular H2A pool in … We would like to show you a description here but the site won’t allow us. hindustan hyundai jaipur contact numberWebThe tumor suppressor BRCA1, also phosphorylated by ATM/ATR kinases, is one of several proteins that colocalize with phospho-H2AX (-H2AX) at sites of active DNA repair. Both the precise mechanism and the purpose of BRCA1 recruitment to … homemade stove for buildingWebOur Histone H2A.X polyclonal, recombinant monoclonal and monoclonal antibodies are developed in Rabbit, Mouse and Goat. These antibodies have been verified by Relative … home made storm windowsWeb8 rows · H2AFX Species Human Location Chr.11: 119093794 -119094294 on GRCh38 … hindustan hydraulics bending machineWebImmunogen: Phosphorylated Human H2AX Peptide Application: Bioimaging (Routinely Tested), Intracellular staining (flow cytometry) (Tested During Development) Vol. Per Test: 5 µl RRID: AB_1645414 Storage Buffer: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide. Regulatory Status: RUO View All Formats homemade stove circ water