Mitotracker red staining protocol

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August undefined, 2024

http://www.bioon.com.cn/protocol/showarticle.asp?newsid=93218 Web9 mrt. 2015 · L7528 LysoTracker® Red DND-99 577 590 NA L12492 LysoTracker® Deep Red 647 668 NA L7533 LysoSensor™ Blue DND-167 373 425 5.1 L7535 LysoSensor™ Green DND-189 443 505 5.2 L7534 LysoSensor™ Green DND-153 442 505 7.5 L7545 LysoSensor™ Yellow/Blue DND-160 (PDMPO) 329, 384 ‡ 440, 540 ‡ 4.2

Mito-Tracker Red CMXRos (线粒体红色荧光探针,活细胞用) - 产 …

WebMitoTracker green (MTG) and nonylacridine orange (NAO) are fluorescent probes which have been commonly used to assess mitochondrial mass. This is based on the assumption that both MTG and NAO accumulate in mitochondria regardless of the mitochondrial transmembrane potential (ΔΨm). WebMitoTracker Deep Red (MTDR) is a relatively non-toxic, carbocyanine-based, far-red, fluorescent probe that is routinely used to chemically mark and visualize mitochondria in living cells. Previously, we used MTDR at low nano-molar concentrations to stain and metabolically fractionate breast cancer cells into Mito-high and Mito-low cell sub … swat gut punch

线粒体功能检测试剂 Thermo Fisher Scientific - CN

WebTbSIR2rp3 GFP (green) localises up and mitochondrion (red; stained with Mitotracker) in bloodstream form T. brucei; DAPI smearing reveals the nucleus both kinetoplast (large … WebMitoTracker® Deep Red FM is a far red-fluorescent dye (abs/em ∼644/665 nm) that stains mitochondria in live cells and can be used for mitochondrial localization. The dye is well-retained after aldehyde fixation and even … swat gta 4 health

Can anybody give me protocol for mitochondria staining using ...

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Webassessed by staining with MitoTracker1 Red and observing with a confocal microscope as described previously.20 Briefly, HepG2 cells were seeded in 12-well plates containing poly-L-lysine coated coverslips at a density of 0.5 106 cells/well. Glass coverslips of 12 mm diameter were acid washed, dried and coated with 50 mg/mL poly-L-lysine (of Web1 aug. 2011 · MitoTracker is a commercially available fluorescent dye (Invitrogen/Molecular Probes) that, like the aforementioned dyes, labels mitochondria … sky and grass cartoonWeb11 mei 2011 · Label the cells with MitoTracker Deep Red 633 for staining mitochondria (100 nm in the culture medium, M-22426, Molecular Probes) and incubate for 30 min while at the CO2 incubator CRITICAL STEP staining for mitochondria should be done in live myocytes as the MitoTracker will stain mitochondria only when it is alive. s.w.a.t gratis

"WebMitoTracker® Red FM is a far red-fluorescent dye (abs/em ∼581/644 nm) that stains mitochondria in live cells and its accumulation is dependent upon membrane potential. For Research Use Only. Not for use in … " - Mitotracker red staining protocol

Mitotracker red staining protocol

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Web13 feb. 2008 · I thought mitotracker was for staining live cells. I didnt know they worked on tissues. You will have to use some mitochondrial antibodies for tissue staining like pyruvate dehydrogenase or some other antibody against protiens in the oxidative phosphorylation. -scolix- QUOTE (scolix @ Feb 13 2008, 12:23 PM) WebIn live tissue MitoTracker ® Red CMXRos and IraZolve-Mito staining and NAD(P)H detection should produce this characteristic mitochondrial staining pattern. The emission intensity from IraZolve-Mito should not decrease over time in tissue samples, and the protocol can be used for prolonged imaging at normal light intensity with minimal …

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WebSample protocol 1. Remove medium from the cells and add prewarmed medium with MitoTracker (most commonly used concentration is in the range of 300-400 nmol). 2. … WebTo overcome this limitation, Molecular Probes has developed MitoTracker probes ― a series of patented mitochondrion-selective stains that are concentrated by active mitochondria and well retained during cell fixation. 16 Because the MitoTracker Orange, MitoTracker Red and MitoTracker Deep Red probes are also retained following …

WebMitotracker Green Fm Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Average 86 stars, based on 1 article reviews. Price from $9.99 to $1999.99. WebA brief protocol as conducted: 1) 50 ug of lyophilized MitoTracker Deep Red was diluted in 100 ul of DMSO to produce 0.92mM stock (kept in -20°C) 2) 100 nM and 200 nM working …

WebLD, MD and HD represent the Se/CDs groups supplemented with 50, 100 and 200 μg/mL of Se/CDs, respectively. MitoTracker represents the MitoTracker staining of different groups. DIC represents the bright field of ovine MII oocytes after MitoTracker staining. MERGED represents the merged results of MitoTracker staining and DIC. Scale bar=50 μm. Web18 aug. 2024 · Cardiomyocytes were stained with Mitotracker Red CMXRos (red) and Hoechst 33342 (blue) for mitochondrial and nuclear staining, respectively. Treatment with 50 μM 4HNE for 24 hours ( A, B ) disrupted mitochondrial network connectivity and Carvedilol improved network connectivity, did not demonstrate a change in mitochondrial …

Web29 nov. 2024 · Trypan blue staining was used to exclude dead cells and to determine cell titers. Approximately 80 × 10 6 paraclonal cells were stained with 25 nM MitoTracker Deep Red for 30 min in cell culture medium at 37 °C. After …

WebMitoTracker探针是一种小的（<1kDa）细胞渗透性线粒体选择性染料，含有巯基反应性氯甲基基序，在固定后仍然维持在线粒体内。因为探针与线粒体硫醇形成共价键，所以它们只能用作终点检测活细胞上样时的线粒体膜电位，而不能实时检测线粒体膜随时间动态的变化。固定剂对MitoTracker探针的影响虽然在研究线粒体结构的分析中可以标记MitoTracker … s.w.a.t gunsWeb1 jan. 2024 · This protocol suggests a quick way of evaluating mitochondrial fitness using flow cytometry. We propose using the probes MitoTraker Green and MitoTracker Red/ … sky and field ruanaWeb29 mei 2024 · The staining protocol proposed is versatile, providing a universal procedure for cell biologists and pathologists to visualise mitochondria. Introduction The normal … swat gunclubWeb14 apr. 2024 · Thus, we reasoned that mitochondrial translation defects would cause disruption of the ETC and loss of mitochondrial membrane potential. To test this, we stained WT and CBFB_KO MCF10A cells with MitoTracker Green and MitoTracker Red, which both stain mitochondria but are membrane potential independent or dependent, … swath a babyWebContribute to carpenter-singh-lab/2024_Cimini_NatureProtocols development by creating an account on GitHub. sky and everest clipartWeb12 apr. 2024 · The medium was removed from the plate and Opti-MEM containing 200 nM MitoTracker® Red CMXRos (M7512, Invitrogen, USA) was added. ... All animal study protocols were approved by the Animal Ethics Committee of the Nanfang Hospital of Southern Medical University ... A–F). Meanwhile, IHC staining revealed that Ki67 … swat gut punch imdbWebMitochondrial membrane potential was measured in wild-type or HILPDA knockout Huh7 cells using MitoTracker Red CMXRos (Invitrogen, M7512) according to the manufacturer’s instructions. Briefly, Huh7 cells were seeded onto 35 mm dishes with glass bottom (MatTek Corporation, P35G-1.5-14-C) at 0.2-0.3x10 6 cells/dish or in a black 96-well plate with … s.w.a.t guest stars